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• Bahşi, Z
• Büyükaksoy, A
• Ölmezcan, S
• Şimşek, F
• Aslan, M
• Oral, A
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• Bahşi, Z
• Büyükaksoy, A
• Ölmezcan, S
• Şimşek, F
• Aslan, M
• Oral, A
• [+] on PubMed
• Bahşi, Z
• Büyükaksoy, A
• Ölmezcan, S
• Şimşek, F
• Aslan, M
• Oral, A

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Sensors 2009, 9(6), 4890-4900; doi:10.3390/s90604890

Article

A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests

Zehra Banu Bahşi ¹ , Aligül Büyükaksoy ² , Sinan Mert Ölmezcan ² , Fethi Şimşek ³ , Muhammed Hasan Aslan ⁴  and Ahmet Yavuz Oral ^2,*

¹ Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey ² Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey ³ Nanotechnology Laboratory, Doga Nanobiotech, Istanbul, Turkey ⁴ Dept of Physics, Gebze Institute of Technology, Kocaeli, Turkey

* Author to whom correspondence should be addressed.

Received: 24 April 2009; in revised form: 10 June 2009 / Accepted: 15 June 2009 / Published: 19 June 2009

(This article belongs to the Section Biosensors)

Download PDF Full-Text [520 KB, uploaded 19 June 2009 06:46 CEST]

Abstract: SiO₂-TiO₂ thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the E. coli O157:H7 EDL933 species using strands of 20-mers (5’-TAATATCGGTTGCGGAGGTG -3’) sequence.

Keywords: sol-gel; optical DNA biosensor; prism coupler

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