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Sensors 2009, 9(6), 4272-4285; https://doi.org/10.3390/s90604272

Coronatine Gene Expression In Vitro and In Planta, and Protein Accumulation During Temperature Downshift in Pseudomonas syringae

1
Jacobs University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
2
National Institute for Medical Research, The Ridgeway, London NW7 1AA, UK
Both authors contributed equally to the work
*
Author to whom correspondence should be addressed.
Received: 1 April 2009 / Revised: 22 May 2009 / Accepted: 26 May 2009 / Published: 3 June 2009
(This article belongs to the Section Biosensors)
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Abstract

The plant pathogenic bacterium Pseudomonas syringae PG4180 synthesizes high levels of the phytotoxin coronatine (COR) at the virulence-promoting temperature of 18 °C, but negligible amounts at 28 °C. Temperature-dependent COR gene expression is regulated by a modified two-component system, consisting of a response regulator, CorR, the histidine protein kinase CorS, and a third component, termed CorP. We analyzed at transcriptional and translational levels the expression of corS and the cma operon involved in COR biosynthesis after a temperature downshift from 28 to 18 °C. Expression of cma was induced within 20 min and increased steadily whereas corS expression was only slightly temperature-dependent. Accumulation of CmaB correlated with accumulation of cma mRNA. However, cma transcription was suppressed by inhibition of de novo protein biosynthesis. A transcriptional fusion of the cma promoter to a promoterless egfp gene was used to monitor the cma expression in vitro and in planta. A steady induction of cma::egfp by temperature downshift was observed in both environments. The results indicate that PG4180 responds to a temperature decrease with COR gene expression. However, COR gene expression and protein biosynthesis increased steadily, possibly reflecting adaptation to long-term rather than rapid temperature changes. View Full-Text
Keywords: COR; coronatine; TCS; two-component system; HPK; histidine protein kinase; RR; response regulator COR; coronatine; TCS; two-component system; HPK; histidine protein kinase; RR; response regulator
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Braun, Y.; Smirnova, A.V.; Weingart, H.; Schenk, A.; Ullrich, M.S. Coronatine Gene Expression In Vitro and In Planta, and Protein Accumulation During Temperature Downshift in Pseudomonas syringae . Sensors 2009, 9, 4272-4285.

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