Sensors 2009, 9(5), 3627-3634; doi:10.3390/s90503627
Article

Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms

Miroslav Pohanka 1, 2,* email, Ladislav Novotný 1 email, Jan Misík 2 email, Kamil Kuca 1, 2 email, Jana Zdarova-Karasova 2  and Martina Hrabinova 1
1 Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic 2 Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
* Author to whom correspondence should be addressed.
Received: 20 April 2009; in revised form: 10 May 2009 / Accepted: 13 May 2009 / Published: 14 May 2009
(This article belongs to the Special Issue Tissue, Cell and Molecular Bioelectrochemistry - New Trends and Approaches)
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Abstract: Cholinesterase activity in blood of laboratory rats was monitored. Rats were intoxicated with paraoxon at dosis of 0 – 65 – 125 – 170 – 250 – 500 nmol. The 250 nmol dose was found to be the LD50. An electrochemical sensor was found useful to provide information about cholinesterase activity. The decrease of cholinesterase activity was correlated to intoxication symptoms and mortality level. It was found that the symptoms of intoxication are not observed while at least 50% of cholinesterase activity in blood remains. The minimal cholinesterase activity essential to survival is around 10%, when compared with the initial state. No changes in levels of low moleculary weight antioxidants were observed.
Keywords: cholinesterase; intoxication; acetylcholinesterase; butyrylcholinesterase; paraoxon; pesticide; activity; blood

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MDPI and ACS Style

Pohanka, M.; Novotný, L.; Misík, J.; Kuca, K.; Zdarova-Karasova, J.; Hrabinova, M. Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms. Sensors 2009, 9, 3627-3634.

AMA Style

Pohanka M, Novotný L, Misík J, Kuca K, Zdarova-Karasova J, Hrabinova M. Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms. Sensors. 2009; 9(5):3627-3634.

Chicago/Turabian Style

Pohanka, Miroslav; Novotný, Ladislav; Misík, Jan; Kuca, Kamil; Zdarova-Karasova, Jana; Hrabinova, Martina. 2009. "Evaluation of Cholinesterase Activities During in Vivo Intoxication Using an Electrochemical Sensor Strip – Correlation With Intoxication Symptoms." Sensors 9, no. 5: 3627-3634.

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