Next Article in Journal
Measurement and Evaluation of Finger Tapping Movements Using Log-linearized Gaussian Mixture Networks
Next Article in Special Issue
Advances in Microbial Biofilm Prevention on Indwelling Medical Devices with Emphasis on Usage of Acoustic Energy
Previous Article in Journal
Electromechanical Characteristic Analysis of Passive Matrix Addressing for Grating Light Modulator
Previous Article in Special Issue
Apparent Thixotropic Properties of Saline/Glycerol Drops with Biotinylated Antibodies on Streptavidin-Coated Glass Slides: Implications for Bacterial Capture on Antibody Microarrays
Article Menu

Export Article

Open AccessArticle
Sensors 2009, 9(3), 2176-2186; doi:10.3390/s90302176

Development of a Novel, Ultra-rapid Biosensor for the Qualitative Detection of Hepatitis B Virus-associated Antigens and Anti-HBV, Based on “Membrane-engineered” Fibroblast Cells with Virus-Specific Antibodies and Antigens

1
Laboratory of Plant Physiology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
2
Hippokration General Hospital, Microbiology Division, Vas. Sofias Av. 114, Athens, Greece
3
EMBIO Diagnostics Project, Nicosia, Cyprus
*
Author to whom correspondence should be addressed.
Received: 3 February 2009 / Revised: 16 March 2009 / Accepted: 25 March 2009 / Published: 25 March 2009
(This article belongs to the Special Issue Pathogen Sensors)
View Full-Text   |   Download PDF [180 KB, uploaded 21 June 2014]   |  

Abstract

A novel miniature cell biosensor detection system for the detection of Hepatis B virus (HBV)-associated antigens and anti-HBV is described. The biosensor is based on “membrane-engineered” Vero fibroblast cells immobilized in an alginate matrix. The membrane-engineering process involved the electroinsertion of anti-HBV specific antibodies (anti-HBs, anti-HBe) or antigens (HBsAg) in the membranes of the Vero cells. The attachment of a homologous antigen to the electroinserted antibody (or, respectively, of the antibody to the electroinserted antigen) triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the Bioelectric Recognition Assay (BERA). The sensor was used for screening 133 clinical blood serum samples according to a double-blind protocol. Considerably higher sensor responses were observed against HBV-positive samples, compared with responses against negative samples or samples positive for heterologous hepatitis viruses such as Hepatitis C (HCV) virus. Detection of anti-HBs antibodies was made possible by using a biosensor based on immobilized Vero cells bearing the respective antigen (HBsAg). The observed response was rapid (45 sec) and quite reproducible. Fluorescence microscopy observations showed that attachment of HBV particles to cells membrane-engineered with anti-HBs was associated with a decrease of [Ca2+]cyt. The perspectives for using the novel biosensor as a qualitative, rapid screening, high throughput assay for HBV antigens and anti-HBs in clinical samples is discussed. View Full-Text
Keywords: Bioelectric Recognition Assay; Cell biosensor; Membrane-engineering; Hepatitis viruses; Vero cells Bioelectric Recognition Assay; Cell biosensor; Membrane-engineering; Hepatitis viruses; Vero cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Perdikaris, A.; Alexandropoulos, N.; Kintzios, S. Development of a Novel, Ultra-rapid Biosensor for the Qualitative Detection of Hepatitis B Virus-associated Antigens and Anti-HBV, Based on “Membrane-engineered” Fibroblast Cells with Virus-Specific Antibodies and Antigens. Sensors 2009, 9, 2176-2186.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top