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• Wang, K
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Sensors 2009, 9(11), 8709-8721; doi:10.3390/s91108709

Article

The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach

Joanne H. Wang¹ , Kevin Wang² , Brandon Bartling³  and Chung-Chiun Liu^3,*

¹ Department of Biology, Brown University, 171 Meeting Street, Providence, RI 02912, USA ² College of Medicine, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA ³ Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA

* Author to whom correspondence should be addressed.

Received: 22 September 2009; in revised form: 15 October 2009 / Accepted: 16 October 2009 / Published: 30 October 2009

(This article belongs to the Section Biosensors)

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Abstract: A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.

Keywords: alkaline phosphatase; electrochemical detection; biosensor

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