Next Article in Journal
HF Radar Sea-echo from Shallow Water
Previous Article in Journal
Water Quality Monitoring for Lake Constance with a Physically Based Algorithm for MERIS Data
Article Menu

Export Article

Open AccessArticle
Sensors 2008, 8(8), 4600-4610; doi:10.3390/s8084600

Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor

1
Instituto de Química, USP, São Paulo, SP, Brazil
2
Instituto de Química de São Carlos, USP, São Carlos, SP, Brazil
*
Author to whom correspondence should be addressed.
Received: 16 June 2008 / Revised: 15 July 2008 / Accepted: 29 July 2008 / Published: 5 August 2008
View Full-Text   |   Download PDF [116 KB, uploaded 21 June 2014]   |  

Abstract

An acetylcholinesterase (AchE) based amperometric biosensor was developed by immobilisation of the enzyme onto a self assembled modified gold electrode. Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirmed the formation of thiocholine and its electrochemical oxidation at Ep = 0.28 V vs Ag/AgCl. An indirect methodology involving the inhibition effect of parathion and carbaryl on the enzymatic reaction was developed and employed to measure both pesticides in spiked natural water and food samples without pre-treatment or pre-concentration steps. Values higher than 91-98.0% in recovery experiments indicated the feasibility of the proposed electroanalytical methodology to quantify both pesticides in water or food samples. HPLC measurements were also performed for comparison and confirmed the values measured amperometrically. View Full-Text
Keywords: Biosensor; Pesticides; Acetylcholinesterase; Creek Water; Food Biosensor; Pesticides; Acetylcholinesterase; Creek Water; Food
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Pedrosa, V.A.; Caetano, J.; Machado, S.A.S.; Bertotti, M. Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor. Sensors 2008, 8, 4600-4610.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top