Next Article in Journal
Previous Article in Journal
Sensors 2008, 8(8), 4600-4610; doi:10.3390/s8084600
Article

Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor

1,* , 2, 2 and 1
Received: 16 June 2008; in revised form: 15 July 2008 / Accepted: 29 July 2008 / Published: 5 August 2008
View Full-Text   |   Download PDF [116 KB, uploaded 21 June 2014]
Abstract: An acetylcholinesterase (AchE) based amperometric biosensor was developed by immobilisation of the enzyme onto a self assembled modified gold electrode. Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirmed the formation of thiocholine and its electrochemical oxidation at Ep = 0.28 V vs Ag/AgCl. An indirect methodology involving the inhibition effect of parathion and carbaryl on the enzymatic reaction was developed and employed to measure both pesticides in spiked natural water and food samples without pre-treatment or pre-concentration steps. Values higher than 91-98.0% in recovery experiments indicated the feasibility of the proposed electroanalytical methodology to quantify both pesticides in water or food samples. HPLC measurements were also performed for comparison and confirmed the values measured amperometrically.
Keywords: Biosensor; Pesticides; Acetylcholinesterase; Creek Water; Food Biosensor; Pesticides; Acetylcholinesterase; Creek Water; Food
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Pedrosa, V.A.; Caetano, J.; Machado, S.A.S.; Bertotti, M. Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor. Sensors 2008, 8, 4600-4610.

AMA Style

Pedrosa VA, Caetano J, Machado SAS, Bertotti M. Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor. Sensors. 2008; 8(8):4600-4610.

Chicago/Turabian Style

Pedrosa, Valber A.; Caetano, Josiane; Machado, Sergio A.S.; Bertotti, Mauro. 2008. "Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor." Sensors 8, no. 8: 4600-4610.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert