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Sensors 2008, 8(7), 4081-4094; doi:10.3390/s8074081
Article

A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction

1, 2, 2, 1,3, 4 and 1,*
Received: 31 May 2008; in revised form: 6 July 2008 / Accepted: 6 July 2008 / Published: 10 July 2008
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Abstract: Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses (50 ng/g or 50 μg/g) of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 μM, in larvae exposed to 50 ng Cd/g it was 2.0 μM and in larvae exposed to 50 μg Cd/g 2.9 μM. Moreover at field study chironomid larvae as well as sediment samples have been collected from eight field sites with different levels of pollution by heavy. The metals content (chromium, nickel, copper, zinc, arsenic, molybdenum, cadmium, tin and lead) in the sediment and or MT content in the chironomid larvae were determined by inductively coupled plasma mass spectrometry or Brdicka reaction, respectively.
Keywords: Catalytic Hydrogen Evolution; Brdicka Reaction; Differential Pulse Voltammetry; Environmental Marker; Thiols; Metallothionein; Heavy Metal Contamination. Catalytic Hydrogen Evolution; Brdicka Reaction; Differential Pulse Voltammetry; Environmental Marker; Thiols; Metallothionein; Heavy Metal Contamination.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Fabrik, I.; Ruferova, Z.; Hilscherova, K.; Adam, V.; Trnkova, L.; Kizek, R. A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction. Sensors 2008, 8, 4081-4094.

AMA Style

Fabrik I, Ruferova Z, Hilscherova K, Adam V, Trnkova L, Kizek R. A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction. Sensors. 2008; 8(7):4081-4094.

Chicago/Turabian Style

Fabrik, Ivo; Ruferova, Zuzana; Hilscherova, Klara; Adam, Vojtech; Trnkova, Libuse; Kizek, Rene. 2008. "A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction." Sensors 8, no. 7: 4081-4094.


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