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• Puida, M
• Ivanauskas, F
• Ignatjev, I
• Valinčius, G
• Razumas, V
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• Puida, M
• Ivanauskas, F
• Ignatjev, I
• Valinčius, G
• Razumas, V
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• Puida, M
• Ivanauskas, F
• Ignatjev, I
• Valinčius, G
• Razumas, V

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Sensors 2008, 8(6), 3873-3879; doi:10.3390/s8063873

Article

Computational Modeling of the Electrochemical System of Lipase Activity Detection

Mantas Puida ^1,* , Feliksas Ivanauskas ¹ , Ilja Ignatjev ² , Gintaras Valinčius ²  and Valdemaras Razumas ²

¹ Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania ² Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania

* Author to whom correspondence should be addressed.

Received: 15 May 2008; in revised form: 31 May 2008 / Accepted: 1 June 2008 / Published: 9 June 2008

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Abstract: This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5’-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process.

Keywords: Amperometric biosensor; lipase activity; ferrocene-based substrate; modeling; simulation

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