Next Article in Journal
Comparing Different Approaches for Mapping Urban Vegetation Cover from Landsat ETM+ Data: A Case Study on Brussels
Previous Article in Journal
Fabrication and Characterization of Silicon Micro-Funnels and Tapered Micro-Channels for Stochastic Sensing Applications
Sensors 2008, 8(6), 3873-3879; doi:10.3390/s8063873
Article

Computational Modeling of the Electrochemical System of Lipase Activity Detection

1,* , 1, 2, 2 and 2
Received: 15 May 2008 / Revised: 31 May 2008 / Accepted: 1 June 2008 / Published: 9 June 2008
View Full-Text   |   Download PDF [188 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5’-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process.
Keywords: Amperometric biosensor; lipase activity; ferrocene-based substrate; modeling; simulation Amperometric biosensor; lipase activity; ferrocene-based substrate; modeling; simulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Puida, M.; Ivanauskas, F.; Ignatjev, I.; Valinčius, G.; Razumas, V. Computational Modeling of the Electrochemical System of Lipase Activity Detection. Sensors 2008, 8, 3873-3879.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert