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The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil
Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, Brazil
Departamento de Química, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil
Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
* Authors to whom correspondence should be addressed.
Received: 29 January 2008; Accepted: 14 February 2008 / Published: 3 March 2008
Abstract: The in situ DNA-damaging capacity of berenil (1) has been investigated usingan electrochemical approach employing double stranded (ds) DNA-modified glassy carbonelectrode biosensors. Electrochemical voltammetric sensing of damage caused by 1 todsDNA was monitored by the appearance of peaks diagnostic of the oxidation of guanineand adenine. When 1 was incorporated directly onto the biosensor surface, DNA damagecould be observed at concentrations of additive as low as 10 μM. In contrast, when thedsDNA-modified biosensor was exposed to 1, in acetate buffer solution, the method wasmuch less sensitive and DNA damage could be detected only in the presence of 100 μMberenil. When mixed solutions of 1 and single stranded (ss) DNA, polyguanylic acid orpolyadenylic acid were submitted to voltammetric study, the oxidation signals of therespective bases decreased in a concentration-dependent manner and the major variation ofthe adenine current peak indicated preferential binding of 1 to adenine. The electrochemical results were in close agreement with those deriving from a differentialscanning calorimetric study of the DNA-berenil complex.
Keywords: Berenil; dsDNA biosensor; Electrochemical voltammetric sensing; DNA damage; Differential scanning calorimetry
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De Abreu, F.C.; De Paula, F.S.; Ferreira, D.C.M.; Nascimento, V.B.; Lopes, J.C.D.; Santos, A.M.C.; Santoro, M.M.; Salas, C.E.; Goulart, M.O.F. The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil. Sensors 2008, 8, 1519-1538.
De Abreu FC, De Paula FS, Ferreira DCM, Nascimento VB, Lopes JCD, Santos AMC, Santoro MM, Salas CE, Goulart MOF. The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil. Sensors. 2008; 8(3):1519-1538.
De Abreu, Fabiane C.; De Paula, Francine S.; Ferreira, Danielle C.M.; Nascimento, Valberes B.; Lopes, Julio C.D.; Santos, Alexandre M.C.; Santoro, Marcelo M.; Salas, Carlos E.; Goulart, Marília O.F. 2008. "The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil." Sensors 8, no. 3: 1519-1538.