Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Sensors 2008, 8(2), 1004-1024; doi:10.3390/s8021004
Review

Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology

Received: 28 January 2008; Accepted: 13 February 2008 / Published: 19 February 2008
(This article belongs to the Special Issue Bioanalysis in Vivo/in Vitro)
Download PDF [1148 KB, uploaded 21 June 2014]
Abstract: Site-specific chemical labeling utilizing small fluorescent molecules is apowerful and attractive technique for in vivo and in vitro analysis of cellular proteins,which can circumvent some problems in genetic encoding labeling by large fluorescentproteins. In particular, affinity labeling based on metal-chelation, advantageous due to thehigh selectivity/simplicity and the small tag-size, is promising, as well as enzymaticcovalent labeling, thereby a variety of novel methods have been studied in recent years.This review describes the advances in chemical labeling of proteins, especially highlightingthe metal-chelation methodology.
Keywords: Chemical labeling; Protein; Fluorescent imaging; Tag; Metal-chelation. Chemical labeling; Protein; Fluorescent imaging; Tag; Metal-chelation.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Soh, N. Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology. Sensors 2008, 8, 1004-1024.

AMA Style

Soh N. Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology. Sensors. 2008; 8(2):1004-1024.

Chicago/Turabian Style

Soh, Nobuaki. 2008. "Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology." Sensors 8, no. 2: 1004-1024.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert