Next Article in Journal
Comparison of a Resonant Mirror Biosensor (IAsys) and a Quartz Crystal Microbalance (QCM) for the Study on Interaction between Paeoniae Radix 801 and Endothelin-1
Next Article in Special Issue
Na+,K+-ATPase as the Target Enzyme for Organic and Inorganic Compounds
Previous Article in Journal
Objective Error Criterion for Evaluation of Mapping Accuracy Based on Sensor Time-of-Flight Measurements
Previous Article in Special Issue
Real-time Monitoring of Non-specific Toxicity Using a Saccharomyces cerevisiae Reporter System
Article Menu

Export Article

Open AccessArticle
Sensors 2008, 8(12), 8262-8274; doi:10.3390/s8128262

Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1

SensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, Cape Town, South Africa
Author to whom correspondence should be addressed.
Received: 19 September 2008 / Revised: 1 December 2008 / Accepted: 2 December 2008 / Published: 15 December 2008
(This article belongs to the Special Issue Toxin Sensors)
View Full-Text   |   Download PDF [702 KB, uploaded 21 June 2014]   |  


An aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor’s differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA–based immunosensors. View Full-Text
Keywords: Immunosensor; Gold nanoparticles; Aflatoxin B1; Polythionine; Horseradish peroxidise (HRP) Immunosensor; Gold nanoparticles; Aflatoxin B1; Polythionine; Horseradish peroxidise (HRP)

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Owino, J.H.; Arotiba, O.A.; Hendricks, N.; Songa, E.A.; Jahed, N.; Waryo, T.T.; Ngece, R.F.; Baker, P.G...; Iwuoha, E.I. Electrochemical Immunosensor Based on Polythionine/Gold Nanoparticles for the Determination of Aflatoxin B1. Sensors 2008, 8, 8262-8274.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top