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Sensors 2008, 8(12), 7894-7903; doi:10.3390/s8127894
Article

Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

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 and 1,*
Received: 7 November 2008; in revised form: 4 December 2008 / Accepted: 5 December 2008 / Published: 5 December 2008
(This article belongs to the Section Biosensors)
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Abstract: The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
Keywords: High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Grieshaber, D.; De Lange, V.; Hirt, T.; Lu, Z.; Vörös, J. Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations. Sensors 2008, 8, 7894-7903.

AMA Style

Grieshaber D, De Lange V, Hirt T, Lu Z, Vörös J. Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations. Sensors. 2008; 8(12):7894-7903.

Chicago/Turabian Style

Grieshaber, Dorothee; De Lange, Victoria; Hirt, Thomas; Lu, Zhihua; Vörös, Janos. 2008. "Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations." Sensors 8, no. 12: 7894-7903.



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