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Sensors 2008, 8(12), 7894-7903; doi:10.3390/s8127894
Article

Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

1
,
1
,
2
,
2
 and
1,*
1 ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland 2 BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA
* Author to whom correspondence should be addressed.
Received: 7 November 2008 / Revised: 4 December 2008 / Accepted: 5 December 2008 / Published: 5 December 2008
(This article belongs to the Section Biosensors)
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Abstract

The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
Keywords: High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Grieshaber, D.; De Lange, V.; Hirt, T.; Lu, Z.; Vörös, J. Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations. Sensors 2008, 8, 7894-7903.

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