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• Grieshaber, D
• De Lange, V
• Hirt, T
• Lu, Z
• Vörös, J
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• De Lange, V
• Hirt, T
• Lu, Z
• Vörös, J
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• De Lange, V
• Hirt, T
• Lu, Z
• Vörös, J

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Sensors 2008, 8(12), 7894-7903; doi:10.3390/s8127894

Article

Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

Dorothee Grieshaber¹ , Victoria De Lange¹ , Thomas Hirt² , Zhihua Lu²  and Janos Vörös^1,*

¹ ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland ² BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA

* Author to whom correspondence should be addressed.

Received: 7 November 2008; in revised form: 4 December 2008 / Accepted: 5 December 2008 / Published: 5 December 2008

(This article belongs to the Section Biosensors)

Download PDF Full-Text [470 KB, uploaded 5 December 2008 14:40 CET]

Abstract: The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.

Keywords: High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance

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