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Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland
BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA
* Author to whom correspondence should be addressed.
Received: 7 November 2008; in revised form: 4 December 2008 / Accepted: 5 December 2008 / Published: 5 December 2008
Abstract: The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.
Keywords: High sensitivity; antigen detection; biosensor; lipid vesicles; quartz crystal microbalance
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MDPI and ACS Style
Grieshaber, D.; De Lange, V.; Hirt, T.; Lu, Z.; Vörös, J. Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations. Sensors 2008, 8, 7894-7903.
Grieshaber D, De Lange V, Hirt T, Lu Z, Vörös J. Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations. Sensors. 2008; 8(12):7894-7903.
Grieshaber, Dorothee; De Lange, Victoria; Hirt, Thomas; Lu, Zhihua; Vörös, Janos. 2008. "Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations." Sensors 8, no. 12: 7894-7903.