Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay

doi:10.3390/s8127571

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Sensors 2008, 8(12), 7571-7580; doi:10.3390/s8127571

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Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay

Madalina Tudorache ¹ and Camelia Bala ^1,2^,*

¹ Laboratory of Quality Control and Process Monitoring, University of Bucharest, 4-12 Regina Elisabeta, 030018 Bucharest, Romania ² Department of Analytical Chemistry, University of Bucharest, 4-12 Regina Elisabeta, 030018 Bucharest, Romania

* Author to whom correspondence should be addressed.

Received: 6 October 2008; in revised form: 28 October 2008 / Accepted: 20 November 2008 / Published: 26 November 2008

(This article belongs to the Section Chemical Sensors)

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Abstract: A magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA) has been developed as new an alternative immunoassay for Aflatoxin B1 determination. The method is based on conventional competitive ELISA whereby the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles’ surface. The influence of the antibody type as well as antibody immobilization on the magnetic beads surface was investigated in detail. Also, optimum values for the general parameters of the method (e.g. tracer concentration, type of antibody, and incubation time) were established. Finally, a sensitive immunoassay method (mp-ELISA) was performed for Aflatoxin B1 determination at ppt level (LOD = 1 ppt Aflatoxin B1).

Keywords: Magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA); Aflatoxin B1; immobilization; antibody; sensitivity

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MDPI and ACS Style

Tudorache, M.; Bala, C. Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay. Sensors 2008, 8, 7571-7580.

AMA Style

Tudorache M., Bala C. Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay. Sensors. 2008; 8(12):7571-7580.

Chicago/Turabian Style

Tudorache, Madalina; Bala, Camelia. 2008. "Sensitive Aflatoxin B1 Determination Using a Magnetic Particles-Based Enzyme-Linked Immunosorbent Assay." Sensors 8, no. 12: 7571-7580.

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