Next Article in Journal
Fabrication and Characterisation of GaAs Gunn Diode Chips for Applications at 77 GHz in Automotive Industry
Previous Article in Journal
High Frequency Quartz Micro Balances: A Promising Path to Enhanced Sensitivity of Gravimetric Sensors
Article Menu

Export Article

Open AccessCommunication
Sensors 2006, 6(4), 341-349; doi:10.3390/S6040341

A Concept for a Sensitive Micro Total Analysis System for High Throughput Fluorescence Imaging

1
Tel-Aviv University, Physical Electronics Department, Ramat-Aviv, Tel-Aviv 69978, Israel
2
School of Applied Chemistry, Waseda University, Step 21, #40 31-103 Titsuka-cho, Shinjuku-ku, Tokyo, Japan
*
Author to whom correspondence should be addressed.
Received: 26 June 2005 / Accepted: 4 January 2006 / Published: 7 April 2006
View Full-Text   |   Download PDF [311 KB, uploaded 20 June 2014]   |  

Abstract

This paper discusses possible methods for on-chip fluorescent imaging forintegrated bio-sensors. The integration of optical and electro-optical accessories, accordingto suggested methods, can improve the performance of fluorescence imaging. It can boostthe signal to background ratio by a few orders of magnitudes in comparison to conventionaldiscrete setups. The methods that are present in this paper are oriented towards buildingreproducible arrays for high-throughput micro total analysis systems (μTAS). The firstmethod relates to side illumination of the fluorescent material placed into micro-compartments of the lab-on-chip. Its significance is in high utilization of excitation energyfor low concentration of fluorescent material. The utilization of a transparent μLED chip,for the second method, allows the placement of the excitation light sources on the sameoptical axis with emission detector, such that the excitation and emission rays are directedcontroversly. The third method presents a spatial filtering of the excitation background.
Keywords: Fluorescence; imaging; lab-on-chip; μfluidic; lenslet array; μLED chip; image sensor; biosensor. Fluorescence; imaging; lab-on-chip; μfluidic; lenslet array; μLED chip; image sensor; biosensor.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rabner, A.; Shacham, Y. A Concept for a Sensitive Micro Total Analysis System for High Throughput Fluorescence Imaging. Sensors 2006, 6, 341-349.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top