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Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination
Department of Hematology, Institute of MDS, Zhongda Hospital, Southeast University Medical College, 87 Dingjia Bridge Hunan Road, Nanjing, 210009, PR China
National Laboratory for Molecular and Biomolecular Electronics (Jian-Xiong Wu Laboratory), Southeast University, Nanjing, 210096, PR China
Department of Chemistry, Changshu College of Jiangsu, Changshu, 215500, PR China
* Author to whom correspondence should be addressed.
Received: 21 May 2004; Accepted: 11 November 2004 / Published: 3 May 2005
Abstract: A novel technique was developed, that was high throughput simultaneousscreening of multiple resistance protein expression based on a protein array system. Themethod combined the advantage of the specificity of enzyme-linked immunosorbentassays with the sensitivity and high throughput of microspot. In this system, the multipleresistance protein arrays were created by spotting the captured antibodies onto the glassslide. The arrays were then incubated with cell samples of leukemia patients. The boundproteins were recognized by biotin-conjugated antibodies and detected by CCD.Experiments demonstrated that three multiple resistance proteins, including Pgp, MRPand BCRP which are members of the ATP-binding-cassette (ABC) superfamily ofmembrane transporters could be simultaneously detected using this new approach.Research work shows the result is coincident with flow cytometry (FCM) (P>0.01). Itprovided a methodology to develop many high-density protein array systems to detect avariety of proteins. The protein arrays will provide a powerful tool to identify theleukemia cell protein expression and rapidly validate their MDR determination.
Keywords: MDR; leukemia cell; P-gp; MRP1; BCRP; protein arrays.
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Cite This Article
MDPI and ACS Style
Du, J.; Chen, B.; Zhang, C.; Xu, X.; Cheng, J.; Gao, F.; Lu, Z. Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination. Sensors 2005, 5, 250-257.
Du J, Chen B, Zhang C, Xu X, Cheng J, Gao F, Lu Z. Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination. Sensors. 2005; 5(4):250-257.
Du, Juan; Chen, Baoan; Zhang, Chunxiu; Xu, Xiaoxing; Cheng, Jian; Gao, Feng; Lu, Zuhong. 2005. "Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination." Sensors 5, no. 4: 250-257.