Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection

doi:10.3390/s30600160

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Sensors 2003, 3(6), 160-165; doi:10.3390/s30600160

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Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH₄ Detection

Dorota G. Bijanowska * , Marek Dawgul and Wladyslaw Torbicz

Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Trojdena 4, 02-109 Warsaw, Poland

* Author to whom correspondence should be addressed.

Received: 16 April 2003 / Accepted: 16 June 2003 / Published: 29 June 2003

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Abstract: In this paper urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) snsitive to ammonium ions were used.Results concerning urea determination by the bisensors in blood plasma and in dialysate show that the biosensors can be applied for urea monitoring in the effluent dialysate. However in the case of pNH₄ based EnFETs a special pre-treatment (dilution with tris/HCl buffer) of the samples is necessary.

Keywords: Urea detection; biosensors; EnFET; enzyme immobilisation

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MDPI and ACS Style

Bijanowska, D.G.; Dawgul, M.; Torbicz, W. Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH₄ Detection. Sensors 2003, 3, 160-165.

AMA Style

Bijanowska D.G., Dawgul M., Torbicz W. Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH₄ Detection. Sensors. 2003; 3(6):160-165.

Chicago/Turabian Style

Bijanowska, Dorota G.; Dawgul, Marek; Torbicz, Wladyslaw. 2003. "Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH₄ Detection." Sensors 3, no. 6: 160-165.

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