Impedance Spectroscopy Technique for DNA Hybridization

doi:10.3390/s31000472

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Sensors 2003, 3(10), 472-479; doi:10.3390/s31000472

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Impedance Spectroscopy Technique for DNA Hybridization

S. Hleli ¹, A. Abdelghani ^1,2^,* and A. Tlili ¹

¹ Unité de Recherche de Physique des Semiconducteurs, IPEST, La Marsa, 2070 Tunis, Tunisia ² Institut National des Sciences Appliquées et de Technologies, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia

* Author to whom correspondence should be addressed.

Received: 4 August 2003 / Accepted: 15 September 2003 / Published: 31 October 2003

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Abstract: The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target. This article describes the use of impedance spectroscopy technique for digoxigeninthiol- labeled ssDNA probe immobilization, anti-digoxigenin binding and targeted DNA hybridization. The analysis of the impedance spectra in terms of equivalents circuits of the gold/electrolyte interface and gold/digoxigenin-thiol-labeled/electrolyte interface is discussed. The DNA hybridization shows a variation in the impedance spectra.

Keywords: Impedance Spectroscopy; DNA; Oligonucleotide

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MDPI and ACS Style

Hleli, S.; Abdelghani, A.; Tlili, A. Impedance Spectroscopy Technique for DNA Hybridization. Sensors 2003, 3, 472-479.

AMA Style

Hleli S., Abdelghani A., Tlili A. Impedance Spectroscopy Technique for DNA Hybridization. Sensors. 2003; 3(10):472-479.

Chicago/Turabian Style

Hleli, S.; Abdelghani, A.; Tlili, A. 2003. "Impedance Spectroscopy Technique for DNA Hybridization." Sensors 3, no. 10: 472-479.

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