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Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer
IT college, East China Normal University, Shanghai 200062, China
Shanghai Teachers University, Shanghai 2000234, China
Shanghai Institute for Medicine Control, Shanghai 200233, China
Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
* Author to whom correspondence should be addressed.
Received: 27 February 2002 / Accepted: 19 March 2002 / Published: 23 April 2002
Abstract: A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective conditions the sensor exhibits excellent sensitivity of 98 nA/M and a linear range of 0.1-6.0 mM. The apparent Michanelis-Menten constant of the sensor is 21 mM. The response time is less than 60 seconds. No apparent change in the response to glucose was observed during one month. Foremost, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electrocatalysis of PB layer to H2O2. The sensor has been applied to detect glucose in human blood serum.
Keywords: Prussian Blue; Electrocatalysis; Chitosan; Selective permeability; Glucose sensor
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MDPI and ACS Style
Zhu, J.; Zhu, Z.; Lai, Z.; Wang, R.; Guo, X.; Wu, X.; Zhang, G.; Zhang, Z.; Wang, Y.; Chen, Z. Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer. Sensors 2002, 2, 127-136.
Zhu J, Zhu Z, Lai Z, Wang R, Guo X, Wu X, Zhang G, Zhang Z, Wang Y, Chen Z. Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer. Sensors. 2002; 2(4):127-136.
Zhu, Jianzhong; Zhu, Ziqiang; Lai, Zongsheng; Wang, Rong; Guo, Xiaoming; Wu, Xiaqin; Zhang, Guoxiong; Zhang, Zongrang; Wang, Yiting; Chen, Zongyou. 2002. "Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer." Sensors 2, no. 4: 127-136.