Next Article in Journal
Asymptotic Regional Boundary Observer in Distributed Parameter Systems via Sensors Structures
Previous Article in Journal
Remote Water Temperature Measurements Based on Brillouin Scattering with a Frequency Doubled Pulsed Yb:doped Fiber Amplifier
Sensors 2002, 2(4), 127-136; doi:10.3390/s20400127
Article

Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer

1, 1, 1, 2, 2, 2, 2, 2, 3 and 4
Received: 27 February 2002; Accepted: 19 March 2002 / Published: 23 April 2002
Download PDF [118 KB, uploaded 20 June 2014]
Abstract: A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective conditions the sensor exhibits excellent sensitivity of 98 nA/M and a linear range of 0.1-6.0 mM. The apparent Michanelis-Menten constant of the sensor is 21 mM. The response time is less than 60 seconds. No apparent change in the response to glucose was observed during one month. Foremost, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electrocatalysis of PB layer to H2O2. The sensor has been applied to detect glucose in human blood serum.
Keywords: Prussian Blue; Electrocatalysis; Chitosan; Selective permeability; Glucose sensor Prussian Blue; Electrocatalysis; Chitosan; Selective permeability; Glucose sensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Zhu, J.; Zhu, Z.; Lai, Z.; Wang, R.; Guo, X.; Wu, X.; Zhang, G.; Zhang, Z.; Wang, Y.; Chen, Z. Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer. Sensors 2002, 2, 127-136.

AMA Style

Zhu J, Zhu Z, Lai Z, Wang R, Guo X, Wu X, Zhang G, Zhang Z, Wang Y, Chen Z. Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer. Sensors. 2002; 2(4):127-136.

Chicago/Turabian Style

Zhu, Jianzhong; Zhu, Ziqiang; Lai, Zongsheng; Wang, Rong; Guo, Xiaoming; Wu, Xiaqin; Zhang, Guoxiong; Zhang, Zongrang; Wang, Yiting; Chen, Zongyou. 2002. "Planar Amperometric Glucose Sensor Based on Glucose Oxidase Immobilized by Chitosan Film on Prussian Blue Layer." Sensors 2, no. 4: 127-136.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert