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Sensors 2018, 18(9), 3007; https://doi.org/10.3390/s18093007

Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device

1
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan
2
Department of Applied Chemistry, Faculty of Science and Engineering, Toyo University, Saitama 350-8585, Japan
3
Graduate School of Material Science, University of Hyogo, Hyogo 678-1297, Japan
4
JST, PRESTO, The University of Tokyo, Tokyo 153-8902, Japan
*
Author to whom correspondence should be addressed.
Received: 3 August 2018 / Revised: 31 August 2018 / Accepted: 31 August 2018 / Published: 8 September 2018
(This article belongs to the Special Issue Sensors for Cell Analysis)
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Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophoresis (DEP) device comprising saw-shaped electrodes. Upon application of an electric field, HL-60 cells as models of promyelocytes aggregated and floated between the saw-shaped electrodes, while UE7T-13 cells as models of BMSCs were effectively captured on the tips of the saw-shaped electrodes. After washing out the HL-60 cells from the device selectively, the purity of the UE7T-13 cells was increased from 33% to 83.5% within 5 min. Although further experiments and optimization are required, these results show the potential of the DEP device as a label-free rapid cell isolation system yielding high purity for rare and precious cells such as BMSCs. View Full-Text
Keywords: dielectrophoresis; stem cell; cell enrichment; label-free separation dielectrophoresis; stem cell; cell enrichment; label-free separation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yoshioka, J.; Ohsugi, Y.; Yoshitomi, T.; Yasukawa, T.; Sasaki, N.; Yoshimoto, K. Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device. Sensors 2018, 18, 3007.

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