SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics
AbstractWe have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein–protein interactions (PPI) as well as protein–peptide or protein–small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin. View Full-Text
Share & Cite This Article
Florinskaya, A.; Ershov, P.; Mezentsev, Y.; Kaluzhskiy, L.; Yablokov, E.; Medvedev, A.; Ivanov, A. SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics. Sensors 2018, 18, 1616.
Florinskaya A, Ershov P, Mezentsev Y, Kaluzhskiy L, Yablokov E, Medvedev A, Ivanov A. SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics. Sensors. 2018; 18(5):1616.Chicago/Turabian Style
Florinskaya, Anna; Ershov, Pavel; Mezentsev, Yuri; Kaluzhskiy, Leonid; Yablokov, Evgeniy; Medvedev, Alexei; Ivanov, Alexis. 2018. "SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics." Sensors 18, no. 5: 1616.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.