Next Article in Journal
Pure FPGA Implementation of an HOG Based Real-Time Pedestrian Detection System
Previous Article in Journal
Optimized Multi-Spectral Filter Array Based Imaging of Natural Scenes
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Sensors 2018, 18(4), 1173; doi:10.3390/s18041173

Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection

1
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia
2
Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor D.E., Malaysia
*
Author to whom correspondence should be addressed.
Received: 2 March 2018 / Revised: 30 March 2018 / Accepted: 2 April 2018 / Published: 12 April 2018
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [8176 KB, uploaded 12 April 2018]   |  

Abstract

A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N,N′-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 × 10−16–1.0 × 10−10 M (R2 = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes. View Full-Text
Keywords: dengue virus detection; nickel(II) salphen complex; optical DNA biosensor; porous silica nanospheres; reflectance measurement; synthetic DNA binder dengue virus detection; nickel(II) salphen complex; optical DNA biosensor; porous silica nanospheres; reflectance measurement; synthetic DNA binder
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Ariffin, E.Y.; Tan, L.L.; Abd. Karim, N.H.; Yook Heng, L. Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection. Sensors 2018, 18, 1173.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top