Sensors 2018, 18(4), 1161; https://doi.org/10.3390/s18041161
Label-Free QCM Immunosensor for the Detection of Ochratoxin A
Şerife Şeyda Pirinçci 1,2,†,*
, Özlem Ertekin 1,†,*
, Duygu Ercan Laguna 1,‡, Fehime Şeyma Özen 1,‡, Zafer Ziya Öztürk 3 and Selma Öztürk 1
, Özlem Ertekin 1,†,*
, Duygu Ercan Laguna 1,‡, Fehime Şeyma Özen 1,‡, Zafer Ziya Öztürk 3 and Selma Öztürk 11
TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey
2
Department of Medical Genetics and Molecular Biology, Kocaeli University, Umuttepe, Kocaeli 41100, Turkey
3
Department of Physics, Gebze Technical University, Gebze, Kocaeli 41400, Turkey
†
These authors contributed equally to this work.
‡
The two authors are out of work now.
*
Authors to whom correspondence should be addressed.
Received: 3 February 2018 / Revised: 5 March 2018 / Accepted: 8 March 2018 / Published: 11 April 2018
(This article belongs to the Special Issue I3S 2017 Selected Papers)
Abstract
Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. View Full-TextKeywords:
OTA; Ochratoxin A; QCM; quartz crystal microbalance; immunobiosensor; regeneration; immobilization
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Pirinçci, Ş.Ş.; Ertekin, Ö.; Laguna, D.E.; Özen, F.Ş.; Öztürk, Z.Z.; Öztürk, S. Label-Free QCM Immunosensor for the Detection of Ochratoxin A. Sensors 2018, 18, 1161.
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