Label-Free QCM Immunosensor for the Detection of Ochratoxin A
AbstractOchratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 165 KB)
Share & Cite This Article
Pirinçci, Ş.Ş.; Ertekin, Ö.; Laguna, D.E.; Özen, F.Ş.; Öztürk, Z.Z.; Öztürk, S. Label-Free QCM Immunosensor for the Detection of Ochratoxin A. Sensors 2018, 18, 1161.
Pirinçci ŞŞ, Ertekin Ö, Laguna DE, Özen FŞ, Öztürk ZZ, Öztürk S. Label-Free QCM Immunosensor for the Detection of Ochratoxin A. Sensors. 2018; 18(4):1161.Chicago/Turabian Style
Pirinçci, Şerife Ş.; Ertekin, Özlem; Laguna, Duygu E.; Özen, Fehime Ş.; Öztürk, Zafer Z.; Öztürk, Selma. 2018. "Label-Free QCM Immunosensor for the Detection of Ochratoxin A." Sensors 18, no. 4: 1161.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.