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Sensors 2018, 18(2), 385; https://doi.org/10.3390/s18020385

A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins

1,2,†
,
1,2,†
,
1,2
,
1,2
,
1,2,* and 2,3
1
Department of Chemistry, Tsinghua University, Beijing 100084, China
2
The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
3
School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 29 December 2017 / Revised: 23 January 2018 / Accepted: 25 January 2018 / Published: 29 January 2018
(This article belongs to the Special Issue Carbon Materials Based Sensors and the Application)
View Full-Text   |   Download PDF [4971 KB, uploaded 29 January 2018]   |  

Abstract

The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells. View Full-Text
Keywords: peptide; GO; ariginines; sensitivity; selectivity peptide; GO; ariginines; sensitivity; selectivity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shi, H.; Zhang, B.; Liu, S.; Tan, C.; Tan, Y.; Jiang, Y. A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins. Sensors 2018, 18, 385.

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