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Sensors 2018, 18(2), 357; https://doi.org/10.3390/s18020357

Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China
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Author to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 26 December 2017 / Accepted: 22 January 2018 / Published: 26 January 2018
(This article belongs to the Special Issue Environmental Monitoring Biosensors)
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Abstract

For the convenience of fast measurement in the outdoor environment, a portable electrochemiluminescence biosensor with the screen-printed electrode as the reaction center was developed, which possesses the characteristics of high sensitivity, small scale, simplified operation and so on, and has been used for in situ detection of abrin. First, combining with magnetic separation technique, the “biotin-avidin” method was used to immobilize the polyclonal antibody (pcAb) on the magnetic microspheres surface as the capture probe. Secondly, the Ru(bpy)32+-labeled monoclonal antibody (mcAb) was used as the specific electrochemiluminescence signal probe. Then, the “mcAb-toxin-pcAb” sandwich model was built to actualize the quantitative detection of abrin on the surface of the screen-printed electrode. The linear detection range was 0.5–1000 ng/mL; the regression equation was Y = 89.251lgX + 104.978 (R = 0.9989, n = 7, p < 0.0001); and the limit of detection (LOD) was 0.1 ng/mL. The sensing system showed high sensitivity, excellent specificity and good anti-interference ability, and could be used for the analysis of trace abrin in various environmental samples with good recovery and reproducibility. Compared with the traditional electrochemiluminescence sensing device, its miniaturization and portability gives it potential to satisfy the requirement of in situ detection. View Full-Text
Keywords: screen printed electrode; electrochemiluminescence; portable sensor; abrin screen printed electrode; electrochemiluminescence; portable sensor; abrin
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Liu, S.; Tong, Z.; Mu, X.; Liu, B.; Du, B.; Liu, Z.; Gao, C. Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode. Sensors 2018, 18, 357.

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