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Sensors 2018, 18(2), 354; https://doi.org/10.3390/s18020354

Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy

Sensors and Biosensors Group, Department of Chemistry, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain
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Received: 20 December 2017 / Revised: 16 January 2018 / Accepted: 23 January 2018 / Published: 26 January 2018
(This article belongs to the Special Issue I3S 2017 Selected Papers)
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Abstract

This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN)6]3−/[Fe(CN)6]4− as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 µM for lysozyme and a limit of detection of 1.67 µM. The sensitivity of the established method was 0.090 µM−1 in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis. View Full-Text
Keywords: aptamer; biosensor; electrochemical impedance spectroscopy; electrochemical grafting aptamer; biosensor; electrochemical impedance spectroscopy; electrochemical grafting
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ortiz-Aguayo, D.; del Valle, M. Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy. Sensors 2018, 18, 354.

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