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Sensors 2018, 18(1), 85; doi:10.3390/s18010085

A Field-Portable Cell Analyzer without a Microscope and Reagents

1
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea
2
Maritime Safety Research Division, Korea Research Institute of Ships & Ocean Engineering, Daejeon 34103, Korea
3
Department of Electro-Mechanical Systems Engineering, Korea University, Sejong 30019, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 26 December 2017 / Accepted: 28 December 2017 / Published: 29 December 2017
(This article belongs to the Special Issue Point of Care Sensors)
View Full-Text   |   Download PDF [3400 KB, uploaded 29 December 2017]   |  

Abstract

This paper demonstrates a commercial-level field-portable lens-free cell analyzer called the NaviCell (No-stain and Automated Versatile Innovative cell analyzer) capable of automatically analyzing cell count and viability without employing an optical microscope and reagents. Based on the lens-free shadow imaging technique, the NaviCell (162 × 135 × 138 mm3 and 1.02 kg) has the advantage of providing analysis results with improved standard deviation between measurement results, owing to its large field of view. Importantly, the cell counting and viability testing can be analyzed without the use of any reagent, thereby simplifying the measurement procedure and reducing potential errors during sample preparation. In this study, the performance of the NaviCell for cell counting and viability testing was demonstrated using 13 and six cell lines, respectively. Based on the results of the hemocytometer (de facto standard), the error rate (ER) and coefficient of variation (CV) of the NaviCell are approximately 3.27 and 2.16 times better than the commercial cell counter, respectively. The cell viability testing of the NaviCell also showed an ER and CV performance improvement of 5.09 and 1.8 times, respectively, demonstrating sufficient potential in the field of cell analysis. View Full-Text
Keywords: lens-free cell analyzer; automatic cell analysis algorithm; reagent-free; cell counting; viability analysis; Lens-Free Shadow Imaging Technology (LSIT) lens-free cell analyzer; automatic cell analysis algorithm; reagent-free; cell counting; viability analysis; Lens-Free Shadow Imaging Technology (LSIT)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Seo, D.; Oh, S.; Lee, M.; Hwang, Y.; Seo, S. A Field-Portable Cell Analyzer without a Microscope and Reagents. Sensors 2018, 18, 85.

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