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Sensors 2018, 18(1), 55; doi:10.3390/s18010055

Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays

1
Medical-Device Lab, Electronics and Telecommunications Research Institute, Daejeon 305-700, Korea
2
Department of Electronics Engineering, Incheon National University, Incheon 22012, Korea
3
Hyper-connected Basic Research Lab, Electronics and Telecommunications Research Institute, Daejeon 305-700, Korea
*
Author to whom correspondence should be addressed.
Received: 12 October 2017 / Revised: 1 December 2017 / Accepted: 23 December 2017 / Published: 26 December 2017
(This article belongs to the Special Issue Novel Approaches to Biosensing with Nanoparticles)
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Abstract

Using gold nanoparticles (AuNPs) on “capillary enzyme-linked immunosorbent assay (ELISA)”, we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of AuNPs, AuNPs of varying diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm, and 50 nm) conjugated with Horseradish Peroxidase (HRP)-labeled anti-C reactive protein (antiCRP) (AuNP•antiCRP-HRP) were used for well-plate ELISA. AuNP of 10 nm produced the largest optical density, enabling detection of 0.1 ng/mL of CRP with only 30 s of incubation, in contrast to 10 ng/mL for the ELISA run in the absence of AuNP. Then, AuNP of 10 nm conjugated with antiCRP-HRP (AuNP•antiCRP-HRP) was used for “capillary ELISA” to detect as low as 0.1 ng/mL of CRP. Also, kinetic study on both 96-well plates and in a capillary tube using antiCRP-HRP or AuNP•antiCRP-HRP showed a synergistic effect between AuNP and the capillary system, in which the fastest assay was observed from the “AuNP capillary ELISA”, with its maximum absorbance reaching 2.5 min, while the slowest was the typical well-plate ELISA with its maximum absorbance reaching in 13.5 min. View Full-Text
Keywords: capillary ELISA; ImmunoGold conjugate; C-reactive protein (CRP); point-of-care (POC) capillary ELISA; ImmunoGold conjugate; C-reactive protein (CRP); point-of-care (POC)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kim, W.-J.; Cho, H.Y.; Jeong, B.; Byun, S.; Huh, J.; Kim, Y.J. Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays. Sensors 2018, 18, 55.

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