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Sensors 2017, 17(9), 2116; https://doi.org/10.3390/s17092116

Ultra-Sensitive NT-proBNP Quantification for Early Detection of Risk Factors Leading to Heart Failure

Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea
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Received: 21 August 2017 / Revised: 8 September 2017 / Accepted: 12 September 2017 / Published: 14 September 2017
(This article belongs to the Special Issue Protein-Based Biosensors)
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Abstract

Cardiovascular diseases such as acute myocardial infarction and heart failure accounted for the death of 17.5 million people (31% of all global deaths) in 2015. Monitoring the level of circulating N-terminal proBNP (NT-proBNP) is crucial for the detection of people at risk of heart failure. In this article, we describe a novel ultra-sensitive NT-proBNP test (us-NT-proBNP) that allows the quantification of circulating NT-proBNP in 30 min at 25 °C in the linear detection range of 7.0–600 pg/mL. It is a first report on the application of a fluorescence bead labeled detection antibody, DNA-guided detection method, and glass fiber membrane platform for the quantification of NT-proBNP in clinical samples. Limit of blank, limit of detection, and limit of quantification were 2.0 pg/mL, 3.7 pg/mL, and 7 pg/mL, respectively. The coefficient of variation was found to be less than 10% in the entire detection range of 7–600 pg/mL. The test demonstrated specificity for NT-proBNP without interferences from bilirubin, intra-lipid, biotin, and hemoglobin. The serial dilution test for plasma samples containing various NT-proBNP levels showed the linear decrement in concentration with the regression coefficient of 0.980–0.998. These results indicate that us-NT-proBNP test does not suffer from the interference of the plasma components for the measurement of NT-proBNP in clinical samples. View Full-Text
Keywords: NT-proBNP; heart failure; left ventricular hypertrophy; 9G DNAChip; DAGON; cardiovascular disease NT-proBNP; heart failure; left ventricular hypertrophy; 9G DNAChip; DAGON; cardiovascular disease
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Song, K.-S.; Nimse, S.B.; Sonawane, M.D.; Warkad, S.D.; Kim, T. Ultra-Sensitive NT-proBNP Quantification for Early Detection of Risk Factors Leading to Heart Failure. Sensors 2017, 17, 2116.

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