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Sensors 2017, 17(9), 2012; https://doi.org/10.3390/s17092012

Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method

1
Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea
2
Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Korea
*
Author to whom correspondence should be addressed.
Received: 31 July 2017 / Revised: 19 August 2017 / Accepted: 31 August 2017 / Published: 2 September 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
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Abstract

A simple method of nanoparticle decoration can be used in the detection of pneumococcus. After the pneumococcal bacteria were captured by an antibody (pneumococcal C-polysaccharide (PnC) antibody) between the interdigitated electrodes, the gold nanoparticles conjugated with the PnC antibodies were let to bind onto an outer membrane of the bacteria. Upon successfully dense decoration, the bacteria surface will become conductive owing to the metal nanoparticles, and a distinctive conductance change between the electrodes can be observed. Since this success ratio, or the probability of the conductance change, reflects the concentration of the analyte, a number of repeated measurements can be used in the quantification of the bacteria. In this way, we have successfully detected S. pneumoniae in the range of 10–108 CFU/mL. The limit of detection in this work is lower than that in the commercial detection kit. We hope that the nanoparticle decoration method will play a role in the facile detection of various bacteria. View Full-Text
Keywords: pathogen detection; bacteria; microgap; biosensors; nanoparticle decoration pathogen detection; bacteria; microgap; biosensors; nanoparticle decoration
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Pyo, H.; Lee, C.Y.; Kim, D.; Kim, G.; Lee, S.; Yun, W.S. Electrical Detection of Pneumococcus through the Nanoparticle Decoration Method. Sensors 2017, 17, 2012.

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