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Sensors 2017, 17(9), 1984; doi:10.3390/s17091984

Development and Validation of a Reproducible and Label-Free Surface Plasmon Resonance Immunosensor for Enrofloxacin Detection in Animal-Derived Foods

Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China
Author to whom correspondence should be addressed.
Received: 3 July 2017 / Revised: 5 August 2017 / Accepted: 28 August 2017 / Published: 30 August 2017
(This article belongs to the Special Issue Surface Plasmon Resonance Sensing)
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This study describes the development of a reproducible and label-free surface plasmon resonance (SPR) immunosensor and its application in the detection of harmful enrofloxacin (ENRO) in animal-derived foods. The experimental parameters for the immunosensor construction and regeneration, including the pH value (4.5), concentration for coating ENRO-ovalbumin conjugate (ENRO-OVA) (100 μg·mL−1), concentration of anti-ENRO antibody (80 nM) and regeneration solution (0.1 mol·L−1 HCl) were evaluated in detail. With the optimized parameters, the proposed SPR immunosensor obtained a good linear response to ENRO with high sensitivity (IC50: 3.8 ng·mL−1) and low detection limit (IC15: 1.2 ng·mL−1). The proposed SPR immunosensor was further validated to have favorable performances for ENRO residue detection in typical animal-derived foods after a simple matrix pretreatment procedure, as well as acceptable accuracy (recovery: 84.3–96.6%), precision (relative standard deviation (n = 3): 1.8–4.6%), and sensitivity (IC15 ≤ 8.4 ng·mL−1). Each SPR chip for analysis can be reused at least 100 times with good stability and the analysis cycle containing the steps of sample uploading/chip regeneration/baseline recovery can be completed within 6 min (one cycle) and auto-operated by a predetermined program. These results demonstrated that the proposed SPR immunosensor provided an effective strategy for accurate, sensitive, and rapid detection for ENRO residue, which has great potential for routine analysis of large numbers of samples for measuring different types of compounds. View Full-Text
Keywords: enrofloxacin; surface plasmon resonance; immunosensor; animal-derived food samples enrofloxacin; surface plasmon resonance; immunosensor; animal-derived food samples

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pan, M.; Li, S.; Wang, J.; Sheng, W.; Wang, S. Development and Validation of a Reproducible and Label-Free Surface Plasmon Resonance Immunosensor for Enrofloxacin Detection in Animal-Derived Foods. Sensors 2017, 17, 1984.

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