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Sensors 2017, 17(8), 1819; doi:10.3390/s17081819

Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment

1
Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, Korea
2
School of Electrical Engineering, Korea University, Seoul 02841, Korea
3
Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 10326, Korea
4
Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 July 2017 / Revised: 3 August 2017 / Accepted: 5 August 2017 / Published: 7 August 2017
(This article belongs to the Special Issue Sensors for Health Monitoring and Disease Diagnosis)
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Abstract

A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 × 100 µm2 (length × width) sized cantilever was released by a 5 µm gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to Aβ peptide. First, the quantification of Aβ peptide with a concentration of 10 pg/mL to 1 μg/mL was performed. The resonant responses exhibited a dynamic range from 100 pg/mL to 100 ng/mL (−56.5 to −774 ΔHz) and a dissociation constant (KD) of binding affinity was calculated as 1.75 nM. Finally, the Aβ self-aggregation associated with AD pathogenesis was monitored by adding monomeric Aβ peptides. As the concentration of added analyte increased from 100 ng/mL to 10 µg/mL, both the frequency shift values (−813 to −1804 ΔHz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate. View Full-Text
Keywords: cantilever sensor; biosensor; viscous damping; Alzheimer’s disease cantilever sensor; biosensor; viscous damping; Alzheimer’s disease
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chae, M.-S.; Kim, J.; Yoo, Y.K.; Lee, J.H.; Kim, T.G.; Hwang, K.S. Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment. Sensors 2017, 17, 1819.

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