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Sensors 2017, 17(8), 1801; https://doi.org/10.3390/s17081801

Genotyping and Bio-Sensing Chemosensory Proteins in Insects

1
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
2
Protein Engineering and Functionality Unit, University of Nantes, Nantes 44322, France
3
QILU University of Technology, School of Bioengineering, Jinan 250353, China
*
Author to whom correspondence should be addressed.
Received: 24 May 2017 / Revised: 28 July 2017 / Accepted: 29 July 2017 / Published: 4 August 2017
(This article belongs to the Special Issue Genosensing)
View Full-Text   |   Download PDF [1454 KB, uploaded 17 August 2017]   |  

Abstract

Genotyping is the process of determining differences in the genetic make-up of an individual and comparing it to that of another individual. Focus on the family of chemosensory proteins (CSPs) in insects reveals differences at the genomic level across various strains and biotypes, but none at the level of individuals, which could be extremely useful in the biotyping of insect pest species necessary for the agricultural, medical and veterinary industries. Proposed methods of genotyping CSPs include not only restriction enzymatic cleavage and amplification of cleaved polymorphic sequences, but also detection of retroposons in some specific regions of the insect chromosome. Design of biosensors using CSPs addresses tissue-specific RNA mutations in a particular subtype of the protein, which could be used as a marker of specific physiological conditions. Additionally, we refer to the binding properties of CSP proteins tuned to lipids and xenobiotic insecticides for the development of a new generation of biosensor chips, monitoring lipid blood concentration and chemical environmental pollution. View Full-Text
Keywords: biotype imprinting; lipometer; mutation sensor; RNA editing; genosensing biotype imprinting; lipometer; mutation sensor; RNA editing; genosensing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, G.; Arnaud, P.; Offmann, B.; Picimbon, J.-F. Genotyping and Bio-Sensing Chemosensory Proteins in Insects. Sensors 2017, 17, 1801.

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