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Sensors 2017, 17(8), 1724; doi:10.3390/s17081724

Evaluation of Shifted Excitation Raman Difference Spectroscopy and Comparison to Computational Background Correction Methods Applied to Biochemical Raman Spectra

1
Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9, Jena 07743, Germany
2
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, Jena 07743, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 26 June 2017 / Revised: 18 July 2017 / Accepted: 20 July 2017 / Published: 27 July 2017
(This article belongs to the Special Issue Applications of Raman Spectroscopy in Biosensors)
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Abstract

Raman spectroscopy provides label-free biochemical information from tissue samples without complicated sample preparation. The clinical capability of Raman spectroscopy has been demonstrated in a wide range of in vitro and in vivo applications. However, a challenge for in vivo applications is the simultaneous excitation of auto-fluorescence in the majority of tissues of interest, such as liver, bladder, brain, and others. Raman bands are then superimposed on a fluorescence background, which can be several orders of magnitude larger than the Raman signal. To eliminate the disturbing fluorescence background, several approaches are available. Among instrumentational methods shifted excitation Raman difference spectroscopy (SERDS) has been widely applied and studied. Similarly, computational techniques, for instance extended multiplicative scatter correction (EMSC), have also been employed to remove undesired background contributions. Here, we present a theoretical and experimental evaluation and comparison of fluorescence background removal approaches for Raman spectra based on SERDS and EMSC. View Full-Text
Keywords: Raman spectroscopy; SERDS; EMSC; background correction; signal to noise ratio Raman spectroscopy; SERDS; EMSC; background correction; signal to noise ratio
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MDPI and ACS Style

Cordero, E.; Korinth, F.; Stiebing, C.; Krafft, C.; Schie, I.W.; Popp, J. Evaluation of Shifted Excitation Raman Difference Spectroscopy and Comparison to Computational Background Correction Methods Applied to Biochemical Raman Spectra. Sensors 2017, 17, 1724.

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