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Sensors 2017, 17(7), 1652; doi:10.3390/s17071652

Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode

1
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
2
Institute of Biotechnology, Henan University, Kaifeng 475000, China
3
Department of Ophthalmology and Visual Sciences and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI 53726, USA
4
Institute of Biochemistry and Biophysics, University of Tehran, Enquelab Avenue, Tehran 13145-1384, Iran
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 8 June 2017 / Revised: 5 July 2017 / Accepted: 13 July 2017 / Published: 18 July 2017
(This article belongs to the Special Issue Advanced Sensors Based on Carbon Electrodes)
View Full-Text   |   Download PDF [1962 KB, uploaded 18 July 2017]   |  

Abstract

A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA. View Full-Text
Keywords: aminated reduced graphene oxide; adenine; guanine; electrochemical detection; glassy carbon electrode aminated reduced graphene oxide; adenine; guanine; electrochemical detection; glassy carbon electrode
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Li, D.; Yang, X.-L.; Xiao, B.-L.; Geng, F.-Y.; Hong, J.; Sheibani, N.; Moosavi-Movahedi, A.A. Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode. Sensors 2017, 17, 1652.

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