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Sensors 2017, 17(7), 1639; doi:10.3390/s17071639

Electrochemical Flow-ELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System

1
Department of Biotechnology, Lund University, Box 124, SE-22100 Lund, Sweden
2
Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Private Bag 16, Plot 10071, Palapye, Botswana
3
CapSenze Biosystems AB, Scheelevägen 22, SE-22363 Lund, Sweden
*
Author to whom correspondence should be addressed.
Received: 6 June 2017 / Revised: 30 June 2017 / Accepted: 5 July 2017 / Published: 16 July 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [2839 KB, uploaded 16 July 2017]   |  

Abstract

An amperometric immunoanalysis system based on monoclonal antibodies immobilized on Sepharose beads and packed into a micro-immunocolumn was developed for the quantification of microcystin-LR. Microcystin-LR (MCLR) was used as a reference microcystin variant. Inside the immunocolumn, free microcystins and microcystin-horseradish peroxidase (tracer) were sequentially captured by the immobilized antibodies, and the detection was performed electrochemically using Super AquaBlue ELISA substrate 2,2′-azinobis(3-ethylbenzothiazoline-sulfonic acid) (ABTS). The ABTS●+ generated by enzymatic oxidation of ABTS was electrochemically determined at a carbon working electrode by applying a reduction potential set at 0.4 V versus Ag/AgCl reference electrode. The peak current intensity was inversely proportional to the amount of analyte bound to the immunocolumn. The amperometric flow-ELISA system, which was automatically controlled through the CapSenzeTM (Lund, Sweden) computer software, enabled determination of MCLR as low as 0.01 µg/L. The assay time was very short (20 min for one assay cycle). In addition, the electrochemical signals were not significantly affected by possible interferences which could be present in the real samples. Along with the simplicity of automation, this makes the developed method a promising tool for use in water quality assessment. View Full-Text
Keywords: microcystin-LR; flow-ELISA; amperometric biosensor; sequential injection; detection microcystin-LR; flow-ELISA; amperometric biosensor; sequential injection; detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lebogang, L.; Jantra, J.; Hedström, M.; Mattiasson, B. Electrochemical Flow-ELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System. Sensors 2017, 17, 1639.

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