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Sensors 2017, 17(7), 1618; https://doi.org/10.3390/s17071618

Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD+ on NiO Film Modified by GO and MBs

1
Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
2
Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
3
Department of Information and Electronic Commerce Management, TransWorld University, Douliu 64063, Taiwan
4
Graduate School of Mechanical Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan
*
Author to whom correspondence should be addressed.
Received: 30 May 2017 / Revised: 1 July 2017 / Accepted: 7 July 2017 / Published: 12 July 2017
(This article belongs to the Section Biosensors)
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Abstract

We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ( NAD + ) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By using GO and MBs, it exhibits excellent sensitivity (45.397 mV/mM) with a linearity of 0.992 in a range of 0.2 mM to 3 mM. According to the results of electrochemical impedance spectroscopy (EIS), the electron transfer resistance of LDH- NAD + -MBs/GPTS/GO/NiO film was smaller than those of LDH-NAD+/GPTS/GO/NiO film and LDH- NAD + /GPTS/NiO film, and it presented the outstanding electron transfer ability. After that, the limit of detection, anti-interference effect and bending test were also investigated. View Full-Text
Keywords: lactate biosensor; nickel oxide (NiO); graphene oxide (GO); magnetic beads (MBs); electrochemical impedance spectroscopy (EIS) lactate biosensor; nickel oxide (NiO); graphene oxide (GO); magnetic beads (MBs); electrochemical impedance spectroscopy (EIS)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chou, J.-C.; Yan, S.-J.; Liao, Y.-H.; Lai, C.-H.; Wu, Y.-X.; Wu, C.-Y.; Chen, H.-Y.; Huang, H.-Y.; Wu, T.-Y. Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD+ on NiO Film Modified by GO and MBs. Sensors 2017, 17, 1618.

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