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Sensors 2017, 17(7), 1480; doi:10.3390/s17071480

Adenosine Triphosphate-Encapsulated Liposomes with Plasmonic Nanoparticles for Surface Enhanced Raman Scattering-Based Immunoassays

1
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea
2
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea
3
Department of Chemistry Education, Seoul National University, Seoul 151-742, Korea
*
Author to whom correspondence should be addressed.
Received: 22 May 2017 / Revised: 21 June 2017 / Accepted: 21 June 2017 / Published: 23 June 2017
(This article belongs to the Special Issue Novel Approaches to Biosensing with Nanoparticles)
View Full-Text   |   Download PDF [2076 KB, uploaded 23 June 2017]   |  

Abstract

In this study, we prepared adenosine triphosphate (ATP) encapsulated liposomes, and assessed their applicability for the surface enhanced Raman scattering (SERS)-based assays with gold-silver alloy (Au@Ag)-assembled silica nanoparticles (NPs; SiO2@Au@Ag). The liposomes were prepared by the thin film hydration method from a mixture of l-α-phosphatidylcholine, cholesterol, and PE-PEG2000 in chloroform; evaporating the solvent, followed by hydration of the resulting thin film with ATP in phosphate-buffered saline (PBS). Upon lysis of the liposome, the SERS intensity of the SiO2@Au@Ag NPs increased with the logarithm of number of ATP-encapsulated liposomes after lysis in the range of 8 × 106 to 8 × 1010. The detection limit of liposome was calculated to be 1.3 × 10−17 mol. The successful application of ATP-encapsulated liposomes to SiO2@Au@Ag NPs based SERS analysis has opened a new avenue for Raman label chemical (RCL)-encapsulated liposome-enhanced SERS-based immunoassays. View Full-Text
Keywords: adenosine triphosphate encapsulated liposomes; plasmonic nanoparticles; gold-silver alloys; surface enhanced Raman scattering adenosine triphosphate encapsulated liposomes; plasmonic nanoparticles; gold-silver alloys; surface enhanced Raman scattering
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Pham, X.-H.; Hahm, E.; Kim, T.H.; Kim, H.-M.; Lee, S.H.; Lee, Y.-S.; Jeong, D.H.; Jun, B.-H. Adenosine Triphosphate-Encapsulated Liposomes with Plasmonic Nanoparticles for Surface Enhanced Raman Scattering-Based Immunoassays. Sensors 2017, 17, 1480.

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