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Sensors 2017, 17(6), 1459; doi:10.3390/s17061459

Light Emitting Marker for Robust Vision-Based On-The-Spot Bacterial Growth Detection

1
Urban Robotics Laboratory, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea
2
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea
*
Author to whom correspondence should be addressed.
Received: 2 April 2017 / Revised: 6 June 2017 / Accepted: 19 June 2017 / Published: 21 June 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
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Abstract

Simple methods using the striped pattern paper marker and FFT (fast Fourier transformation) have been proposed as alternatives to measuring the optical density for determining the level of bacterial growth. The marker-based method can be easily automated, but due to image-processing-base of the method, the presence of light or the color of the culture broth can disturb the detection process. This paper proposes a modified version of marker-FFT-based growth detection that uses a light emitting diode (LED) array as a marker. Since the marker itself can emit the light, the measurements can be performed even when there is no light source or the bacteria are cultured in a large volume of darkly colored broth. In addition, an LED marker can function as a region of interest (ROI) indicator in the image. We expect that the proposed LED-based marker system will allow more robust growth detection compared to conventional methods. View Full-Text
Keywords: bacterial growth; light emitting marker; LED marker; fast Fourier transformation; robust growth detection bacterial growth; light emitting marker; LED marker; fast Fourier transformation; robust growth detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, K.; Hyun, J.; Jeon, J.S. Light Emitting Marker for Robust Vision-Based On-The-Spot Bacterial Growth Detection. Sensors 2017, 17, 1459.

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