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Sensors 2017, 17(6), 1375; doi:10.3390/s17061375

Microcontact Imprinted Plasmonic Nanosensors: Powerful Tools in the Detection of Salmonella paratyphi

1
Department of Biology, Hacettepe University, 06800 Ankara, Turkey
2
Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey
3
Department of Biotechnology and Molecular Biology, Aksaray University, 68100 Aksaray, Turkey
4
Department of Biotechnology, Lund University, 223 62 Lund, Sweden
5
CapSenze Biosystems AB, 223 63 Lund, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Giovanna Marrazza
Received: 12 April 2017 / Revised: 9 May 2017 / Accepted: 31 May 2017 / Published: 13 June 2017
(This article belongs to the Special Issue Biosensors and Molecular Imprinting)
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Abstract

Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106–15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies. View Full-Text
Keywords: microcontact imprinting; SPR biosensor; N-methacryloyl-l-histidine methyl ester; Salmonella paratyphi microcontact imprinting; SPR biosensor; N-methacryloyl-l-histidine methyl ester; Salmonella paratyphi
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Perçin, I.; Idil, N.; Bakhshpour, M.; Yılmaz, E.; Mattiasson, B.; Denizli, A. Microcontact Imprinted Plasmonic Nanosensors: Powerful Tools in the Detection of Salmonella paratyphi. Sensors 2017, 17, 1375.

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