Rapid Waterborne Pathogen Detection with Mobile Electronics
AbstractPathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal–oxide–semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens. View Full-Text
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Wu, T.-F.; Chen, Y.-C.; Wang, W.-C.; Kucknoor, A.S.; Lin, C.-J.; Lo, Y.-H.; Yao, C.-W.; Lian, I. Rapid Waterborne Pathogen Detection with Mobile Electronics. Sensors 2017, 17, 1348.
Wu T-F, Chen Y-C, Wang W-C, Kucknoor AS, Lin C-J, Lo Y-H, Yao C-W, Lian I. Rapid Waterborne Pathogen Detection with Mobile Electronics. Sensors. 2017; 17(6):1348.Chicago/Turabian Style
Wu, Tsung-Feng; Chen, Yu-Chen; Wang, Wei-Chung; Kucknoor, Ashwini S.; Lin, Che-Jen; Lo, Yu-Hwa; Yao, Chun-Wei; Lian, Ian. 2017. "Rapid Waterborne Pathogen Detection with Mobile Electronics." Sensors 17, no. 6: 1348.
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