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Sensors 2017, 17(6), 1316; doi:10.3390/s17061316

Design and Fabrication of a Ratiometric Planar Optode for Simultaneous Imaging of pH and Oxygen

Key Lab of Submarine Geosciences and Prospecting Techniques, Ministry of Education, College of Marine Geosciences, Ocean University of China, Qingdao 266100, China
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Academic Editor: Sheshanath Bhosale
Received: 9 April 2017 / Revised: 10 May 2017 / Accepted: 27 May 2017 / Published: 7 June 2017
(This article belongs to the Special Issue Fluorescent Probes and Sensors)
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Abstract

This paper presents a simple, high resolution imaging approach utilizing ratiometric planar optode for simultaneous measurement of dissolved oxygen (DO) and pH. The planar optode comprises a plastic optical film coated with oxygen indicator Platinum(II) octaethylporphyrin (PtOEP) and reference quantum dots (QDs) embedded in polystyrene (PS), pH indicator 5-Hexadecanoylamino-fluorescein (5-Fluorescein) embedded in Hydromed D4 matrix. The indicator and reference dyes are excited by utilizing an LED (Light Emitting Diode) source with a central wavelength of 405 nm, the emission respectively matches the different channels (red, green, and blue) of a 3CCD camera after eliminating the excitation source by utilizing the color filter. The result shows that there is low cross-sensitivity between the two analytes dissolved oxygen and pH, and it shows good performance in the dynamic response ranges of 0–12 mg/L and a dynamic range of pH 6−8. The optode has been tested with regard to the response times, accuracy, photostability and stability. The applied experiment for detecting pH/Oxygen of sea-water under the influence of the rain drops is demonstrated. It is shown that the planar optode measuring system provides a simple method with low cross-talk for pH/Oxygen imaging in aqueous applications. View Full-Text
Keywords: pH; dissolved oxygen; planar optode; ratiometric; sea-water; rain drops pH; dissolved oxygen; planar optode; ratiometric; sea-water; rain drops
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jiang, Z.; Yu, X.; Hao, Y. Design and Fabrication of a Ratiometric Planar Optode for Simultaneous Imaging of pH and Oxygen. Sensors 2017, 17, 1316.

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