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Sensors 2017, 17(5), 961; doi:10.3390/s17050961

Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe

1
Liver Research Center, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center of Digestive Diseases, 95 Yong-an Road, Xicheng District, Beijing 100050, China
2
Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100190, China
3
Beijing Entry_Exit Inspection and Quarantine Bureau, 3 Yi, Tianzhu donglu, Tianzhu Airport Industry Area, Shunyi District, Beijing 101312, China
4
Wuhan Wawasye Nanotech Company, No. 36 Xinjiangongmen Road, Beijing 100191, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: María Jesús Lobo-Castañón
Received: 27 February 2017 / Revised: 20 April 2017 / Accepted: 21 April 2017 / Published: 26 April 2017
(This article belongs to the Special Issue Genosensing)
View Full-Text   |   Download PDF [1476 KB, uploaded 26 April 2017]   |  

Abstract

Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy. We also evaluated the efficiency of this method for the detection of drug-resistant mutations compared with direct sequencing. In CHB, HBV DNA from the serum samples of patients with poor response or virological breakthrough can be hybridized to probes containing the M204I mutation to visualize fluorescence under fluorescence microscopy, where fluorescence intensity is related to the virus load, in our method. At present, the limits of the method used to detect HBV genetic variations by fluorescence quantum dots is 103 IU/mL. These results show that QDs can be used as fluorescent probes to detect viral HBV DNA polymerase gene variation, and is a simple readout system without complex and expensive instruments, which provides an attractive platform for the detection of HBV M204I mutation. View Full-Text
Keywords: quantum dot; HBV DNA; sequencing; mutation quantum dot; HBV DNA; sequencing; mutation
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Zhang, C.; Chen, Y.; Liang, X.; Zhang, G.; Ma, H.; Nie, L.; Wang, Y. Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe. Sensors 2017, 17, 961.

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