Next Article in Journal
Nitride-Based Materials for Flexible MEMS Tactile and Flow Sensors in Robotics
Previous Article in Journal
Development of Conductivity Sensors for Multi-Phase Flow Local Measurements at the Polytechnic University of Valencia (UPV) and University Jaume I of Castellon (UJI)
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(5), 1079; doi:10.3390/s17051079

GryphSens: A Smartphone-Based Portable Diagnostic Reader for the Rapid Detection of Progesterone in Milk

BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 9 April 2017 / Revised: 5 May 2017 / Accepted: 7 May 2017 / Published: 10 May 2017
(This article belongs to the Section Chemical Sensors)
View Full-Text   |   Download PDF [3187 KB, uploaded 10 May 2017]   |  

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a popular assay technique for the detection and quantification of various biological substances due its high sensitivity and specificity. More often, it requires large and expensive laboratory instruments, which makes it difficult to conduct when the tests must be performed quickly at the point-of-care (POC). To increase portability and ease of use, we propose a portable diagnostic system based on a Raspberry Pi imaging sensor for the rapid detection of progesterone in milk samples. We designed, assembled, and tested a standalone portable diagnostic reader and validated it for progesterone detection against a standard ELISA assay using a commercial plate reader. The portable POC device yielded consistent results, regardless of differences in the cameras and flashlights between various smartphone devices. An Android application was built to provide front-end access to users, control the diagnostic reader, and display and store the progesterone measurement on the smartphone. The diagnostic reader takes images of the samples, reads the pixel values, processes the results, and presents the results on the handheld device. The proposed POC reader can perform to superior levels of performance as a plate reader, while adding the desirable qualities of portability and ease of use. View Full-Text
Keywords: progesterone detection; immunoassay; image analysis; point-of-care reader; android application progesterone detection; immunoassay; image analysis; point-of-care reader; android application
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jang, H.; Ahmed, S.R.; Neethirajan, S. GryphSens: A Smartphone-Based Portable Diagnostic Reader for the Rapid Detection of Progesterone in Milk. Sensors 2017, 17, 1079.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top