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Sensors 2017, 17(5), 1078; doi:10.3390/s17051078

Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection

1
School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China
2
College of Resource and Environment science, Xinjiang University, Urumqi 830046, China
3
College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China
*
Author to whom correspondence should be addressed.
Academic Editor: Philip J. Hill
Received: 4 April 2017 / Revised: 27 April 2017 / Accepted: 6 May 2017 / Published: 10 May 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [4996 KB, uploaded 10 May 2017]   |  

Abstract

A novel assembled biosensor was prepared for detecting 16S rRNA, a small-size persistent specific for Actinobacteria. The mechanism of the porous silicon (PS) photonic crystal biosensor is based on the fluorescence resonance energy transfer (FRET) between quantum dots (QDs) and gold nanoparticles (AuNPs) through DNA hybridization, where QDs act as an emission donor and AuNPs serve as a fluorescence quencher. Results showed that the photoluminescence (PL) intensity of PS photonic crystal was drastically increased when the QDs-conjugated probe DNA was adhered to the PS layer by surface modification using a standard cross-link chemistry method. The PL intensity of QDs was decreased when the addition of AuNPs-conjugated complementary 16S rRNA was dropped onto QDs-conjugated PS. Based on the analysis of different target DNA concentration, it was found that the decrease of the PL intensity showed a good linear relationship with complementary DNA concentration in a range from 0.25 to 10 μM, and the detection limit was 328.7 nM. Such an optical FRET biosensor functions on PS-based photonic crystal for DNA detection that differs from the traditional FRET, which is used only in liquid. This method will benefit the development of a new optical FRET label-free biosensor on Si substrate and has great potential in biochips based on integrated optical devices. View Full-Text
Keywords: FRET; DNA biosensor; porous silicon; photonic crystal FRET; DNA biosensor; porous silicon; photonic crystal
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Zhang, H.; Lv, J.; Jia, Z. Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection. Sensors 2017, 17, 1078.

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