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Sensors 2017, 17(5), 1074; doi:10.3390/s17051074

Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites/Chitosan Nanoparticles

1
Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran 1435116471, Iran
2
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 1435116471, Iran
3
Faculty of Chemistry, Bu-Ali Sina University, Hamedan 6517838695, Iran
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 7 April 2017 / Revised: 2 May 2017 / Accepted: 3 May 2017 / Published: 9 May 2017
(This article belongs to the Section Biosensors)
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Abstract

In this work, a novel nanocomposite consisting of electrosynthesized gold nanodendrites and chitosan nanoparticles (AuNDs/CSNPs) has been prepared to fabricate an impedimetric immunosensor based on a screen printed carbon electrode (SPCE) for the rapid and sensitive immunoassay of botulinum neurotoxin A (BoNT/A). BoNT/A polyclonal antibody was immobilized on the nanocomposite-modified SPCE for the signal amplification. The structure of the prepared nanocomposite was investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The charge transfer resistance (RCT) changes were used to detect BoNT/A as the specific immuno-interactions at the immunosensor surface that efficiently limited the electron transfer of Fe(CN)63−/4− as a redox probe at pH = 7.4. A linear relationship was observed between the %∆RCT and the concentration logarithm of BoNT/A within the range of 0.2 to 230 pg·mL−1 with a detection limit (S/N = 3) of 0.15 pg·mL−1. The practical applicability of the proposed sensor was examined by evaluating the detection of BoNT/A in milk and serum samples with satisfactory recoveries. Therefore, the prepared immunosensor holds great promise for the fast, simple and sensitive detection of BoNT/A in various real samples. View Full-Text
Keywords: label-free immunosensor; botulinum neurotoxin; biosensors; gold nanodendrites; chitosan nanoparticles; screen printed carbon electrode label-free immunosensor; botulinum neurotoxin; biosensors; gold nanodendrites; chitosan nanoparticles; screen printed carbon electrode
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sorouri, R.; Bagheri, H.; Afkhami, A.; Salimian, J. Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites/Chitosan Nanoparticles. Sensors 2017, 17, 1074.

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