Next Article in Journal
Multiple Objects Fusion Tracker Using a Matching Network for Adaptively Represented Instance Pairs
Next Article in Special Issue
Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe
Previous Article in Journal
Device-Free Localization via an Extreme Learning Machine with Parameterized Geometrical Feature Extraction
Previous Article in Special Issue
Electrochemical Genosensing of Circulating Biomarkers
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(4), 881; doi:10.3390/s17040881

A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops

1
Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain
2
Departamento de Química, Centro de Ciências da Natureza, Universidade Federal do Piauí, Teresina 64049-550PI, Brazil
3
Instituto de Ciencias Biológicas, Universidade de Pernambuco, Recife 50670-901PE, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Nicole Jaffrezic-Renault
Received: 14 March 2017 / Revised: 10 April 2017 / Accepted: 12 April 2017 / Published: 18 April 2017
(This article belongs to the Special Issue Genosensing)
View Full-Text   |   Download PDF [2150 KB, uploaded 20 April 2017]   |  

Abstract

The design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein. The proposed test allows for the determination of a transgene copy number in both hemizygous (maize MON810 trait) and homozygous (soybean GTS40-3-2) transformed plants, and exhibits a limit of quantification of at least 0.25% for both kinds of GMO lines. View Full-Text
Keywords: electrochemical genosensor; genetically modified crops; PCR; screening GMOs electrochemical genosensor; genetically modified crops; PCR; screening GMOs
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Moura-Melo, S.; Miranda-Castro, R.; de-los-Santos-Álvarez, N.; Miranda-Ordieres, A.J.; dos Santos Junior, J.R.; da Silva Fonseca, R.A.; Lobo-Castañón, M.J. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors 2017, 17, 881.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top