A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops
AbstractThe design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein. The proposed test allows for the determination of a transgene copy number in both hemizygous (maize MON810 trait) and homozygous (soybean GTS40-3-2) transformed plants, and exhibits a limit of quantification of at least 0.25% for both kinds of GMO lines. View Full-Text
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Moura-Melo, S.; Miranda-Castro, R.; de-los-Santos-Álvarez, N.; Miranda-Ordieres, A.J.; dos Santos Junior, J.R.; da Silva Fonseca, R.A.; Lobo-Castañón, M.J. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors 2017, 17, 881.
Moura-Melo S, Miranda-Castro R, de-los-Santos-Álvarez N, Miranda-Ordieres AJ, dos Santos Junior JR, da Silva Fonseca RA, Lobo-Castañón MJ. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops. Sensors. 2017; 17(4):881.Chicago/Turabian Style
Moura-Melo, Suely; Miranda-Castro, Rebeca; de-los-Santos-Álvarez, Noemí; Miranda-Ordieres, Arturo J.; dos Santos Junior, José R.; da Silva Fonseca, Rosana A.; Lobo-Castañón, María J. 2017. "A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops." Sensors 17, no. 4: 881.
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