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Sensors 2017, 17(4), 803; doi:10.3390/s17040803

In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl

1
Biotechnology Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266, Sinjeong-Dong, Jeongeup, Jeonbuk 580-185, Korea
2
Department of Textile Engineering and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan, Gyeongbuk 38541, Korea
3
Department of Chemistry, Maharishi Markandeshwar University, Mullana, (Ambala) Haryana 133207, India
4
Ministry of Employment and Labor, Major Industrial Accident Prevention Center, 34 Yeosusandallo, Yeosu-Si, Jeonnam 59631, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 1 December 2016 / Revised: 20 March 2017 / Accepted: 5 April 2017 / Published: 8 April 2017
(This article belongs to the Section Biosensors)
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Abstract

This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM). The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: (i) Real-time chemo-repellent responses of cell dynamics; (ii) Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; (iii) Minimal consumption of cells and antimicrobial agents; and (iv) Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets. View Full-Text
Keywords: PDMS microfluidic sensor; confocal laser scanning microscope; 1-TDPPO; Pseudomonas aeruginosa PAO1; fluorescence intensity PDMS microfluidic sensor; confocal laser scanning microscope; 1-TDPPO; Pseudomonas aeruginosa PAO1; fluorescence intensity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Roh, C.; Lee, J.; Kinger, M.; Kang, C. In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl. Sensors 2017, 17, 803.

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