Next Article in Journal
A Formaldehyde Sensor Based on Molecularly-Imprinted Polymer on a TiO2 Nanotube Array
Previous Article in Journal
A Polynomial Subset-Based Efficient Multi-Party Key Management System for Lightweight Device Networks
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(4), 676; doi:10.3390/s17040676

Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay

1
Faculty of Chemical Technology, University of Pardubice, Studentska 95, Pardubice CZ-53210, Czech Republic
2
Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove CZ-50001, Czech Republic
3
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-61300, Czech Republic
4
Central European Institute of Technology, Brno University of Technology, Purkynova 123, Brno CZ-61200, Czech Republic
5
Department of Geology and Pedology, Faculty of Forestry and Wood Technology, Mendel University in Brno, Brno CZ-61300, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 1 February 2017 / Revised: 20 March 2017 / Accepted: 22 March 2017 / Published: 24 March 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [5670 KB, uploaded 27 March 2017]   |  

Abstract

Magnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer’s drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) molecule was proposed for assay of the neurotoxic compounds. The prepared nanoparticles were modified by pure AChE and they were used for the measurement anti-Alzheimer’s drug galantamine and carbamate pesticide carbofuran with limit of detection 1.5 µM and 20 nM, respectively. All measurements were carried out using screen-printed sensor with carbon working, silver reference, and carbon auxiliary electrode. Standard Ellman’s assay was used for validation measurement of both inhibitors. Part of this work was the elimination of reversible inhibitors represented by galantamine from the active site of AChE. For this purpose, we used a lower pH to get the original activity of AChE after inhibition by galantamine. We also observed decarbamylation of the AChE-carbofuran adduct. Influence of organic solvents to AChE as well as repeatability of measurement with MPs with AChE was also established. View Full-Text
Keywords: acetylcholinesterase; magnetic particles; electrochemistry; screen-printed sensor; carbofuran; galantamine; nanomaterial; nanoparticles acetylcholinesterase; magnetic particles; electrochemistry; screen-printed sensor; carbofuran; galantamine; nanomaterial; nanoparticles
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kostelnik, A.; Kopel, P.; Cegan, A.; Pohanka, M. Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay. Sensors 2017, 17, 676.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top