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Sensors 2017, 17(11), 2570; doi:10.3390/s17112570

Development of Ratiometric Fluorescent Biosensors for the Determination of Creatine and Creatinine in Urine

School of Chemical Engineering, Research Center for Biophotonics, Chonnam National University, Yong-Bong Ro77, 61186 Gwangju, Korea
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Received: 21 September 2017 / Revised: 23 October 2017 / Accepted: 6 November 2017 / Published: 8 November 2017
(This article belongs to the Special Issue Fluorescent Probes and Sensors)
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Abstract

In this study, the oxazine 170 perchlorate (O17)-ethylcellulose (EC) membrane was successfully exploited for the fabrication of creatine- and creatinine-sensing membranes. The sensing membrane exhibited a double layer of O17-EC membrane and a layer of enzyme(s) entrapped in the EC and polyurethane hydrogel (PU) matrix. The sensing principle of the membranes was based on the hydrolytic catalysis of urea, creatine, and creatinine by the enzymes. The reaction end product, ammonia, reacted with O17-EC membrane, resulting in the change in fluorescence intensities at two emission wavelengths (λem = 565 and 625 nm). Data collected from the ratio of fluorescence intensities at λem = 565 and 625 nm were proportional to the concentrations of creatine or creatinine. Creatine- and creatinine-sensing membranes were very sensitive to creatine and creatinine at the concentration range of 0.1–1.0 mM, with a limit of detection (LOD) of 0.015 and 0.0325 mM, respectively. Furthermore, these sensing membranes showed good features in terms of response time, reversibility, and long-term stability. The interference study demonstrated that some components such as amino acids and salts had some negative effects on the analytical performance of the membranes. Thus, the simple and sensitive ratiometric fluorescent sensors provide a simple and comprehensive method for the determination of creatine and creatinine concentrations in urine. View Full-Text
Keywords: creatine; creatinine; fluorescent sensing membrane; O17-EC membrane; ratiometric calculation creatine; creatinine; fluorescent sensing membrane; O17-EC membrane; ratiometric calculation
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Duong, H.D.; Rhee, J.I. Development of Ratiometric Fluorescent Biosensors for the Determination of Creatine and Creatinine in Urine. Sensors 2017, 17, 2570.

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