Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer’s Disease
AbstractThe clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 323 KB)
Share & Cite This Article
Garyfallou, G.-Z.; Ketebu, O.; Şahin, S.; Mukaetova-Ladinska, E.B.; Catt, M.; Yu, E.H. Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer’s Disease. Sensors 2017, 17, 2464.
Garyfallou G-Z, Ketebu O, Şahin S, Mukaetova-Ladinska EB, Catt M, Yu EH. Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer’s Disease. Sensors. 2017; 17(11):2464.Chicago/Turabian Style
Garyfallou, Goulielmos-Zois; Ketebu, Orlando; Şahin, Samet; Mukaetova-Ladinska, Elizabeta B.; Catt, Michael; Yu, Eileen H. 2017. "Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer’s Disease." Sensors 17, no. 11: 2464.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.